primary antibodies against glial fibrillary acidic protein  (Proteintech)

Journal: Frontiers in Pharmacology

Article Title: Integrating network pharmacology and transcriptomics to reveal the therapeutic effect of Long Mu Ning Xin Decoction on attention-deficit/hyperactivity disorder by regulating cAMP and PI3K/AKT pathways

doi: 10.3389/fphar.2026.1744709

Figure Lengend Snippet: Effects of LMNXD on the relative expression of GFAP and DRD1 positive Area% in the hippocampus and PFC of rats and BDNF protein in the striatum. (A) Immunofluorescence staining of GFAP (Scale: 100um). (B) Immunohistochemical staining of DRD1 (Scale: 200um). (C) Western blotting analysis image of BDNF in the striatum. (D) Relative GFAP positive area%. (E) Relative DRD1 positive area%. (F) Relative expression of BDNF proteins. Data are expressed as mean ± standard deviation, n = 3 in immunohistochemistry and immunofluorescence, n = 6 in Western blot. Compared with the WKY group, # p < 0.05, ## p < 0.01. Compared with the SHR group, * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: Primary antibodies against glial fibrillary acidic protein (GFAP, 60190-1-Ig), dopamine deceptor D1 (DRD1, 17934-1-Ap), and BDNF (66292-1-Ig) were obtained from Proteintech (Wuhan, China).

Techniques: Expressing, Immunofluorescence, Staining, Immunohistochemical staining, Western Blot, Standard Deviation, Immunohistochemistry

Journal: Neural Regeneration Research

Article Title: Positive impact of indicaxanthin from Opuntia ficus-indica fruit on high-fat diet–induced neuronal damage and gut microbiota dysbiosis

doi: 10.4103/NRR.NRR-D-23-02039

Figure Lengend Snippet: Indicaxanthin ameliorates brain inflammation induced by HFD. (A) Transcription levels of iNOS, TNF-α, and IL-6 determined by real-time PCR in the brain. (B) Brain expression of COX-2 and iNOS. (C) Densitometric analysis of COX-2 and iNOS protein levels normalized to β-actin levels. (D) Brain expression of cytosolic p65 and nuclear p65. (E) Densitometric analysis of cytosolic p65 protein levels normalized to β-actin levels and nuclear p65 levels normalized to laminin B levels. (F) Representative images of GFAP-positive cells (red) on the surface. Hoechst staining was used to label the nuclei (blue) (microscope magnification 10×); scale bars: 100 μm. (G) Percentage of GFAP-positive cells. (H, I) Plasma circulating levels of TNF-α (H) and IL-1β (I). Data are presented as the mean ± SEM ( n = 8/group). *** P < 0.001, vs . STD mice; ### P < 0.001, vs. HFD-fed mice. COX-2: Cyclooxygenase-2; GFAP: glial fibrillary acidic protein; HFD: high-fat diet; IL-6: interleukin-6; Ind: indicaxanthin; iNOS: inducible nitric oxide synthase; PCR: polymerase chain reaction; STD: standard diet; TNF-α: tumor necrosis factor-α.

Article Snippet: Next, the sections were incubated with a mouse primary antibody against glial fibrillary acidic protein (GFAP) (1:300, Cell Signaling Technology, Danvers, MA, USA, Cat# 3670, RRID: AB_10693476) at 4°C overnight and then with an anti-mouse IgG-Alexa Fluor 594 secondary antibody (goat, 1:300, Invitrogen, Cat# A-11005, RRID: AB_2534073) for 2 hours at room temperature.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Staining, Microscopy, Clinical Proteomics, Polymerase Chain Reaction

Journal: Animal Models and Experimental Medicine

Article Title: Establishment of a novel alloxan‐induced rabbit model exhibiting unique diabetic retinal neuropathy features assessed via ERG + VEP

doi: 10.1002/ame2.70032

Figure Lengend Snippet: Representative retinal tissue sections stained with H&E from normal and DR rabbits during the 9‐week post‐induction period. (A) H&E‐stained retina tissue sections from control and model rabbits exhibiting retina thickness under a light microscope at 400× magnification. (B) Expression of RBPMS and GFAP in the retina was assessed using IF staining. GCL, Ganglion cell layer; INL, Inner nuclear layer; IPL, Inner plexiform layer; ONL, Outer nuclear layer; OPL, Outer plexiform layer; PRL, Photoreceptor cell layer. * p < 0.05, *** p < 0.0001, **** p < 0.0001.

Article Snippet: Primary antibodies against Glial Fibrillary Acidic Protein (GFAP) (GB11096‐50; Servicebio) and RNA‐binding protein with multiple splicing (RBPMS) ( MG770204 ; Abmart) were incubated overnight at 4°C, followed by a 2‐h incubation with secondary antibodies at room temperature.

Techniques: Staining, Control, Light Microscopy, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Sex Differences in a Novel Mouse Model of Spinocerebellar Ataxia Type 1 (SCA1)

doi: 10.3390/ijms26062623

Figure Lengend Snippet: Sex differences in cerebellar pathology in SCA1 mice. ( A ) Calbindin staining was used to label Purkinje cells in 36-week-old female and male WT and ATXN1 146Q mice. ( B ) Molecular layer width and ( C ) calbindin intensity were analyzed using confocal images. ( D , E ) GFAP staining was ( E ) quantified using confocal images from 36-week-old female and male WT and ATXN1 146Q mice. ( F , G ) Staining with Iba1 was used to quantify microglia in the molecular layer of cerebellum by dividing the number of Iba1 microglia per area in 36-week-old female and male WT and ATXN1 146Q mice ( G ). Scale bar = 50µm. Min of 3 slices from N = 6–8 mice in each condition. Data are an average ± SEM. One-way ANOVA * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001, ns means not significant.

Article Snippet: We used primary antibodies against Purkinje cell marker calbindin (rabbit, Sigma-Aldrich, C9848, St. Louis, MO, USA), astrocytic marker glial fibrillary acidic protein (GFAP) (chicken, Millipore, AB5541, Burlington, MA, USA), and microglial marker ionized calcium-binding adapting molecule 1 (Iba1) (rabbit, Abcam, AB107159, Cambridge, UK) as previously described [ , , ].Confocal images of a minimum of three different slices from each mouse were acquired using a Stellaris microscope (Leica Microsystems Inc., Deerfield, IL, USA) with an oil 20× objective.

Techniques: Staining